Effect of Mango Leaf Shoot Extract (Mangifera indica L.) on Zebrafish (Danio rerio) Cell Regeneration Induced by Hyperglicemia

Adisty Virakawugi Darniwa, Tri Cahyanto, Siti Nurul Hidayah

Abstract


Abstract. In many cases, diabetics often have difficulty in wound healing and lead to amputation. Hyperglycemia is an early symptom of diabetic which is characterized by high blood glucose levels. Cell regeneration is one of part by wound healing process. Mango plants have benefits as medicinal plants because they have secondary metabolites that can help heal wounds. The purpose of this study was to determine the effect of mango leaf shoot extract on blood glucose levels of zebrafish (Danio rerio) hyperglycemia due to alloxan and glucose induction and role of mango leaf shoot extract on zebrafish caudal fin regeneration. This study used an experimental method with 5 group treatments: control group, hyperglycemia, metformin 0,75 w/v, extract 10 µg/ml and extract 20  µg/ml. Parameters observed include blood glucose level, fin length and survival rate. Hyperglycemia was measured by blood glucose level, caudal fin regeneration was analayzed using fin length, also environmental factors and survival rate were measured. Result showed that mango leaf shoot extract 20 µg/ml was efective in reducing blood glucose levels, reaching 68,31% and aproaching metformin 0,75 w/v  treatment with a decrease in blood glucose levels reaching 70,1%. Mango leaf shoot extract with concentration 20 µg/ml significantly different (p < 0,05) from hyperglycemia group and extract 10  µg/ml. Extract 20 µg/ml was effective in assisting the regeneration process with an increase caudal fin length reaching 0.94 cm during the treatment period.

 


Keywords


Hyperglycemia; Mango; Regeneration; Zebrafish

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References


Alasa, A. N., Anam, S., & Jamaluddin, J. (2017). Analisis Kadar Total Metabolit Sekunder Ekstrak Etanol Daun Tamoenju (Hibiscus surattensis L.). Kovalen, 3(3), 258–268.

Alza, Y. (2013). Hubungan Hiperglikemia Dengan Kadar Glyceraldehyde-3-Phosphate Dehydrogenase (Gapdh) Pada Penderita Diabetes Mellitus Tipe 2. Photon: Jurnal Sain Dan Kesehatan, 4(1), 19–26.

American Diabetes Association. (2014). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 37(1), 81–90.

Amiraragab, B., Hussein, S. A., Alm-Eldeen, A.-E., Hafe z, A., & Mohamed, T. (2017). Diabetes Management Saponins and Their Potential Role in Diabetes Mellitus. Diabetes Manag, 7(1), 148–158.

Arip Rahman, Agus Arifin Sentosa, D. W. (2012). Sebaran Ukuran dan Kondisi Ikan Zebra Amatitlania nigrofasciata ( Günther , 1867 ) di Danau Beratan , Bali in Lake Beratan , Bali. Jurnal Iktiologi Indonesia, 12(2), 135–145.

Ayuningtyas, A. (2020). Penggunaan Madu Dan Mangga (Mangifera Indica) Pada Penyembuhan Luka. Journal of Health Science and Physiotherapy, 2(1), 111–115.

Cahyanto, T., Fadillah, A., Ulfa, R. A., Hasby, R. M., & Kinasih, I. (2020). Kadar Mangiferin Pada Lima Kultivar Pucuk Daun Mangga (Mangifera indica L.). Al-Kauniyah: Jurnal Biologi, 13(2), 242–249.

Dar, A., Faizi, S., Naqvi, S., Roome, T., Zikr-Ur-Rehman, S., Ali, M., … Moin, S. T. (2005). Analgesic and antioxidant activity of mangiferin and its derivatives: The structure activity relationship. Biological and Pharmaceutical Bulletin, 28(4), 596–600.

Darniwa, A. V., Cahyanto, T., Kusumorini, A., Meidita, Z., Putri, E., Ulfa, R. A., … Hizqiyah, N. (2020). Uji Perilaku dan Preferensi Area pada Ikan Zebrafish ( Danio rerio ) yang Diinduksi Stres. Biosfer, 5(2), 32–38.

Dinesh, M. R., Hemanth, K. N., Vasanthaiahm K. V., D. (2011). Wild Crop Relatives: Genomic and Breeding Resources. Wild Crop Relatives: Genomic and Breeding Resources, (June).

Djuanda, S. R. S., Novianto, E., Boediardja, S. A., & Jusman, S. W. A. (2012). Peran Stress Oksidatif pada Penuaan Kulit Secara Intrinsik. Mdvi, 39(3), 127–133.

Elza Sundhani, Della Caya Nur Syarifah, Lita Ratriyana Zumrohani, N. A. N. (2016). Efektivitas Ekstrak Etanol Daun Adam Hawa (Rhoeo discolor) dan Daun Pucuk Merah (Syzygium campanulatum Korth.) Dalam Menurunkan Kadar Gula Darah Pada Tikus Putih Jantan Galur Wistar dengan Pembebanan Glukosa. Pharmacy, 13(02), 137–149.

Firdaus, I., Retnowati, R., & Sutrisno. (2015). Fraksinasi Ekstrak metanol Daun Mangga Kasturi (Mangifera casturi Kosterm) Dengan Pelarut n-Butanol. Kimia Student Journal, 1(1), 787–790.

Fithriani, D., Amini, S., Melanie, S., & Susilowati, R. (2015). Uji Fitokimia, Kandungan Total Fenol dan Aktivitas Antioksidan Mikroalga Spirulina sp., Chlorella sp., dan Nannochloropsis sp. Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 10(2), 101–109.

García, A. B. (2017). Brief Update on Diabetes for General Practitioners. Rev Esp Sanid Penit, (19), 57–65.

Gumantara, M. P. B., & Oktarlina, R. Z. (2017). Perbandingan Monoterapi dan Kombinasi Terapi Sulfonilurea-Metformin terhadap Pasien Diabetes Melitus Tipe 2. Majority, 6(1), 55–59.

Hakim, I. R., Lestari, F., & Priani, S. E. (2010). Kajian Pustaka Tanaman yang Berpotensi dalam Penyembuhan Luka Bakar. Prosiding Farmasi, 14–20.

Hardiningtyas, S. D., Purwaningsih, S.-, & Handharyani, E.-. (2014). Aktivitas Antioksidan Dan Efek Hepatoprotektif Daun Bakau Api-Api Putih. Jurnal Pengolahan Hasil Perikanan Indonesia, 17(1), 80–91.

Haryoto, H., & Devi, E. S. (2018). Efek Pemberian Ekstrak Etanol Daun Dan Batang Ubi Jalar Ungu (Ipomoea batatas L.) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Jantan Galur Wistar Yang Diinduksi Aloksan. Talenta Conference Series: Tropical Medicine (TM), 1(3), 139–143.

Hayati, Farida, Putri, Cyntia, A., Awaluddin, R., dkk. (2017). Pengembangan Metode Uji Antihiperglikemia Dengan Zebrafish. Prosiding Seminar Nasional, (7), 476–486.

Heckler, K., & Kroll, J. (2017). Zebrafish as a model for the study of microvascular complications of diabetes and their mechanisms. International Journal of Molecular Sciences, 18(9), 1–9.

Herlina, Nina., Mulyati dan Ananda, P. (2020). Efektivitas Hipoglikemik Fraksi Etil Asetat Ampas Tahu Menggunakan Model Hewan Ikan Zebra (Danio rerio). Jurnal Jamu Indonesia, 5(1), 16–21.

Ighodaro, O. M., Adeosun, A. M., & Akinloye, O. A. (2017). Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina (Lithuania), 53(6), 365–374.

Indrayani, S., & Mustarichie, R. (2020). Aktivitas Antidiabetes Beberapa Tanaman Di Indonesia. Farmaka, 18(1), 58–65.

Ismi, S. (2017). Pengaruh Penggantian Oksigen Pada Transportasi Benih Kerapu Dengan Sistem Tertutup. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 9(1), 385–392.

Juniarti, Y. dan. (2011). Analisis Senyawa Metabolit Sekunder Dari Ekstrak Metanol Daun Surian Yang Berpotensi Sebagai Antioksidan. MAKARA of Science Series, 15(1), 48–52.

Laut, M., Ndaong, N., Utami, T., Junersi, M., Bria Seran, Y., Farmakologi dan Toksikologi FKH Undana Jl Adisucipto, B., … Kedokteran Hewan Jl Adisucipto -Penfui, F. (2019). Efektivitas Pemberian Salep Ekstrak Etanol Daun Anting-anting (Acalypha indica Linn.) Terhadap Kesembuhan Luka Insisi pada Mencit (Mus musculus). Jurnal Kajian Veteriner, 7(1), 1–11.

Luo, Y., Fu, C., Wang, Z., Zhang, Z., Wang, H., & Liu, Y. (2015). Mangiferin attenuates contusive spinal cord injury in rats through the regulation of oxidative stress, inflammation and the Bcl-2 and Bax pathway. Molecular Medicine Reports, 12(5), 7132–7138.

Luqyana Z. T. M, L. dan, & Husni, P. (2019). Aktivitas Farmakologi Tanaman Mangga (Mangifera indica L.): Review. Jurnal Farmaka, 17(2), 187–194.

Matkowski, A., Kus, P., Goralska, E., & Wozniak, D. (2013). Mangiferin – a Bioactive Xanthonoid, not only from Mango and not just Antioxidant. Mini-Reviews in Medicinal Chemistry, 13(3), 439–455.

Medina Ramírez, N., Monteiro Farias, L., Apolonio Santana, F., Viana Leite, J., De Souza Dantas, M., Lopes Toledo, R., … Machado Rocha Ribeiro, S. (2016). Extraction of Mangiferin and Chemical Characterization and Sensorial Analysis of Teas from Mangifera indica L. Leaves of the Ubá Variety. Beverages, 2(4), 33.

Miten, S. V., & Setiasih, L. N. E. S. dan S. K. W. (2014). Pemberian Ekstrak Etanol Kulit Batang Kelor Glukosa Darah Tikus Hiperglikemia. Indonesia Medicus Veterinus, 3(4), 292–299.

Muchdar, F., Juharni, J., & Andriani, R. (2020). Utilization of Different Probiotics on Growth and Survival Rate of Blacktail Zebra fish (Dascyllus melanurus). Agrikan: Jurnal Agribisnis Perikanan, 13(2), 222–231.

Mustofa, Yuniastuti, Ari, dan Marianti, A. (2012). Efek Pemberian Jus Lidah Buaya Terhadap Kadar Glukosa Darah Tikus Putih. Unnes Journal of Life Science, 1(1), 35–40.

Olsen, Ansgar., Sarras, Michael and Intine, R. (2011). Limb Regeneration is Impaired in an Adult Zebrafish Model of Diabetes Mellitus. NIPH Public Access, 23(1), 1–7.

Paredes, L. C., Camara, N. O. S., & Braga, T. T. (2019). Understanding The Metabolic Profile of Macrophages During The Regenerative Process in Zebrafish. Frontiers in Physiology, 10, 1–19.

Permata, E. I., & Khoirunnisa, Y. (2020). Efek Mangiferin dalam Mengatasi Masalah Kesehatan. Jurnal Penelitian Perawat Profesional, 2(1), 31–38.

Permatasari, Sarah, Cahyanto, Tri, Adawiyah, Ayuni dan Ulfa, R. A. (2018). Pucuk Daun Mangga (Mangifera indica L.) Kultivar Cngkir Sebagai Penurun Kadar Glukosa Darah. Bioma : Jurnal Biologi Dan Pembelajaran Biologi, 3(2), 102–112.

Pokorski, M. (2014). Mangiferin - A Nutraceutical with Clinical Implications. Vitamins & Minerals, 03(01), 1–2.

Poss, K. D., Shen, J., Nechiporuk, A., McMahon, G., Thisse, B., Thisse, C., & Keating, M. T. (2000). Roles for Fgf signaling during zebrafish fin regeneration. Developmental Biology, 222(2), 347–358.

Prommajak, T., Kim, S. M., Pan, C. H., Kim, S. M., Surawang, S., & Rattanapanone, N. (2014). Identification of antioxidants in young mango leaves by LC-ABTS and LC-MS. Chiang Mai University Journal of Natural Sciences, 13(3), 317–330.

Risa, A. M., Pantiwati, Y., Mahmudati, N., Husamah, H., & Miharja, F. J. (2018). Daun Mangga (Mangifera indica L): Potensi Baru Penyembuh Luka Sayat. Biota: Biologi Dan Pendidikan Biologi, 11(2), 96–106.

Spampinato, S. F., Caruso, G. I., De Pasquale, R., Sortino, M. A., & Merlo, S. (2020). The Treatment of Impaired Wound Healing in Diabetes: Looking Among Old Drugs. Pharmaceuticals, 13(4), 1–17.

Susanty, S., & Bachmid, F. (2016). Perbandingan Metode Ekstraksi Maserasi dan Refluks Terhadap Kadar Fenolik dari Ekstrak Tongkul Jagung (Zea mays L.). Jurnal Konversi, 5(2), 87–93.

Syaputri, R. R. (2014). Uji Efek Ekstrak Etanol 70% Kulit Buah Asam Jawa. Universitas Muhammadyah Surakarta.

Syarif, R. A., Muhajir, Ahmad, A. R., & Malik, A. (2008). Identifikasi Golongan Senyawa Antioksidan dengan menggunakan Metode Perendaman Radikal DPPH Ekstrak Etanol Daun Cordia myxa L. Jurnal Fitofarmaka Indonesia, 2(1), 83–89.

Szkudelski, T. (2001). The Mechanism of Alloxan and Streptozotocin Action in B Cells of The Rat Pancreas. Physiological Research, 50(6), 537–546.

Tayana, N., Inthakusol, W., Duangdee, N., Chewchinda, S., Pandith, H., & Kongkiatpaiboon, S. (2019). Mangiferin content in different parts of mango tree (Mangifera indica L.) in Thailand. Songklanakarin Journal of Science and Technology, 41(3), 522–528.

Utami, N. (2018). Zebrafish (Danio rerio) Sebagai Hewan Model Diabetes Mellitus. BioTrends, 9(1), 15–19.

Wang, R. R., Gao, Y. D., Ma, C. H., Zhang, X. J., Huang, C. G., Huang, J. F., & Zheng, Y. T. (2011). Mangiferin, an anti-HIV-1 agent targeting protease and effective against resistant strains. Molecules, 16(5), 4264–4277.




DOI: http://dx.doi.org/10.17977/um061v5i22021p43-57

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