Synthesis and In Vitro Testing of Mg-6Zn-xHAp Biocomposites from Beef Bone as Biodegradable Bone Implant Material

Jojor Lamsihar Manalu, Francisca Tjhay, Theodora Kristoforus, Sovian Aritonang

Abstract


This study aimed to develop biodegradable Mg-6Zn hydroxyapatite (Mg-6Zn HAp) biocomposites for potential use in bone replacement applications. The hydroxyapatite (HAp) powders, sourced from cow bone, were synthesized through an eco-friendly and cost-effective process, leveraging bioresources for material sustainability. The Mg-6Zn and HAp powders were mechanically mixed through ball milling for six hours to ensure homogeneity. The resultant powder mixture was then subjected to isostatic pressing at a high pressure of 570 MPa, forming a dense coin-shaped composite with a 1.5 cm diameter. This coin was consolidated in a capsule furnace at elevated temperatures for one hour to enhance material integrity. The Mg-6Zn HAp alloy was thoroughly characterized using X-ray diffraction (XRD) to assess phase formation and crystallographic structure, and Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (SEM-EDX) to examine microstructural features and elemental composition. For composite preparation, varying amounts of HAp (5%, 8%, and 12%) were incorporated into the Mg-6Zn matrix. SEM analyses revealed a uniform distribution of HAp particles along the boundaries of matrix particles, enhancing composite structure and stability. Results demonstrated that with an increase in HAp content, there was a corresponding improvement in the relative density and hardness of the composites. The corrosion rate decreased with higher HAp content, indicating improved biocompatibility and stability in physiological environments. This suggests that the Mg-6Zn HAp biocomposites, with their tailored microstructure and enhanced mechanical properties, hold promise for use in biodegradable bone replacement applications.

Keywords


Biodegradable biocomposites, bone replacement materials, corrosion resistance, MgZnHAp, microstructural characterization

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References


S.G. Sukaryo, A. Purnama, and H. Hermawan, “Structure and properties of biomaterials,” Biomater. Med. Devices a Perspect. from an Emerg. Ctry., pp. 1–22, 2016, doi: 10.1007/978-3-319-14845-8_1.

Q. Chen and G.A. Thouas, “Metallic implant biomaterials,” Mater. Sci. Eng. R Reports, vol. 87, pp. 1–57, 2015.

M. Hussain, S. Ullah, M.R. Raza, N. Abbas, and A. Ali, “Recent developments in Zn-Based biodegradable materials for Biomedical Applications,” J. Funct. Biomater., vol. 14, no. 1, p. 1, 2022.

Y. Liu, X.-H. Chen, J.-A. Yang, H. Pan, D. Chen et al., “Fundamental theory of biodegradable metals—definition, criteria, and design,” Adv. Funct. Mater., vol. 29, no. 18, p. 1805402, 2019.

H. Kabir, K. Munir, C. Wen, and Y. Li, “Recent research and progress of biodegradable zinc alloys and composites for biomedical applications: Biomechanical and biocorrosion perspectives,” Bioact. Mater., vol. 6, no. 3, pp. 836–879, 2021.

T. Zhang, W. Wang, J. Liu, L. Wang, Y. Tang, and K. Wang, “A review on magnesium alloys for biomedical applications,” Front. Bioeng. Biotechnol., vol. 10, p. 953344, 2022.

F. Witte, “The history of biodegradable magnesium implants: a review,” Acta Biomater., vol. 6, no. 5, pp. 1680–1692, 2010.

Y. Chen, J. Yan, Z. Wang, S. Yu, X. Wang, Z. Yuan et al., “In vitro and in vivo corrosion measurements of Mg–6Zn alloys in the bile,” Mater. Sci. Eng. C, vol. 42, pp. 116–123, 2014.

F. Živić, N. Grujović, G. Manivasagam, C. Richard, and J. Landoulsi, “The potential of magnesium alloys as bioabsorbable/biodegradable implants for biomedical applications,” Tribol. Ind., vol. 36, no. 1, p. 67, 2014.

Y. Chen, Z. Xu, C. Smith, and J. Sankar, “Recent advances on the development of magnesium alloys for biodegradable implants,” Acta Biomater., vol. 10, no. 11, pp. 4561–4573, 2014.

X. Gu, Y. Zheng, Y. Cheng, S. Zhong, and T. Xi, “In vitro corrosion and biocompatibility of binary magnesium alloys,” Biomaterials, vol. 30, no. 4, pp. 484–498, 2009.

H. Hermawan, “Biodegradable metals: state of the art,” Biodegrad. Met. From Concept to Appl., pp. 13–22, 2012.

A.H.M. Sanchez, B. J. Luthringer, F. Feyerabend, and R. Willumeit, “Mg and Mg alloys: how comparable are in vitro and in vivo corrosion rates? A review,” Acta Biomater., vol. 13, pp. 16–31, 2015.

T.B. Matias, G.H. Asato, B.T. Ramasco, W.J. Botta, C.S. Kiminami, and C. Bolfarini, “Processing and characterization of amorphous magnesium based alloy for application in biomedical implants,” J. Mater. Res. Technol., vol. 3, no. 3, pp. 203–209, 2014.

D. Vojtěch, J. Kubásek, J. Šerák, and P. Novák, “Mechanical and corrosion properties of newly developed biodegradable Zn-based alloys for bone fixation,” Acta Biomater., vol. 7, no. 9, pp. 3515–3522, 2011.

E.M. Salleh, S. Ramakrishnan, and Z. Hussain, “Synthesis of biodegradable Mg-Zn alloy by mechanical alloying: effect of milling time,” Procedia Chem., vol. 19, pp. 525–530, 2016.

E.M. Salleh, H. Zuhailawati, S. Ramakrishnan, and M.A.H. Gepreel, “A statistical prediction of density and hardness of biodegradable mechanically alloyed Mg–Zn alloy using fractional factorial design,” J. Alloys Compd., vol. 644, pp. 476–484, 2015.

M.B. Kannan, “Hydroxyapatite coating on biodegradable magnesium and magnesium-based alloys,” in Hydroxyapatite (HAp) for biomedical applications, Elsevier, 2015, pp. 289–306.

M. Alizadeh-Osgouei, Y. Li, and C. Wen, “A comprehensive review of biodegradable synthetic polymer-ceramic composites and their manufacture for biomedical applications,” Bioact. Mater., vol. 4, pp. 22–36, 2019.

J.L. Manalu, B. Soegijono, and D.J. Indrani, “Study of Mg-Hydroxyapatite composite with various composition of hydroxyapatite which obtained from cow bones in simulation body fluid (SBF),” Asian J. Appl. Sci., vol. 4, no. 4, 2016.

S. Zhang et al., “In vitro and in vivo corrosion and histocompatibility of pure Mg and a Mg-6Zn alloy as urinary implants in rat model,” Mater. Sci. Eng. C, vol. 68, pp. 414–422, 2016.

S. Biswas, S.S. Dhinwal, and S. Suwas, “Room-temperature equal channel angular extrusion of pure magnesium,” Acta Mater., vol. 58, no. 9, pp. 3247–3261, 2010.

A.K. Khanra, H.C. Jung, S.H. Yu, K.S. Hong, and K.S. Shin, “Microstructure and mechanical properties of Mg-HAP composites,” Bull. Mater. Sci., vol. 33, pp. 43–47, 2010.

K.A. Khalil, “A new-developed nanostructured Mg/HAp nanocomposite by high frequency induction heat sintering process,” in IOP Conference Series: Materials Science and Engineering, 2012, vol. 40, no. 1, p. 12031.

M. Mandal, A.P. Moon, G. Deo, C.L. Mendis, and K. Mondal, “Corrosion behavior of Mg–2.4 Zn alloy micro-alloyed with Ag and Ca,” Corros. Sci., vol. 78, pp. 172–182, 2014.

M. Silalahi, H. Sitompul, J.L. Manalu, K. Dahlan, D. Noviana, and A. Dimyati, “Novel technology on sinthesizing Mg-Zn biomaterial using arc plasma sintering,” Asian J. Appl. Sci., vol. 5, no. 3, 2017.

J. Henny, A. Vassault, G. Boursier, I. Vukasovic, P.M. Brguljan, M. Lohmander et al., “Recommendation for the review of biological reference intervals in medical laboratories,” Clin. Chem. Lab. Med., vol. 54, no. 12, pp. 1893–1900, 2016.




DOI: http://dx.doi.org/10.17977/um016v8i22024p410

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