Study of Surface Morphology and Porosity of Composite Scaffold Nanofiber PVA/CS/HA with Electrospinning Method
Abstract
This research aims to compose nanofibers as a scaffold material in bone tissue engineering in terms of surface morphological properties and porosity. HA nanorod was prepared by the precipitation-ultrasonication method, while the PVA/CS/HA nanofiber composites were made by the electrospinning method using a static collector. HA was characterized by using XRD and SEM-EDX, while the PVA/CS/HA nanofiber composites used FTIR and SEM. The results show that HA nanorod has a crystalline size of 10.86 nm, crystallinity level of 52.38 per cent, and Ca/P ratio of 1.70. From the SEM image shows HA nanorod width of 11.6 nm and 97.53 nm in length and some of it still in the form of HA nanoparticles. The diameter and porosity of PVA/CS/HA nanofiber with addition of 0, 10, 20 per cent HA were 275, 212, 265 nm and 72.94, 69.49, 70.81 per cent, respectively.
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Y. Yudyanto, Y. D. Sugara, and H. Hartatiek, “Pengaruh nanosilika terhadap kekerasan dan porositas nanokomposit HA-SiO2 berbasis batuan onyx Bojonegoro,” JPSE (J. Phys. Sci. Eng.), vol. 1, no. 1, pp. 13–18, 2016.
H. J. Haugen, S. P. Lyngstadaas, F. Rossi, and G. Perale, “Bone grafts: Which is the ideal biomaterial?,” J. Clin. Periodontol., voo. 46, pp. 92–102, 2019.
G. F. de-Grado et al., “Bone substitutes: A review of their characteristics, clinical use, and perspectives for large bone defects management,” J. Tissue Eng., vol. 9. pp. 1–18, 2018.
T. Winkler, F. A. Sass, G. N. Duda, and K. Schmidt-Bleek, “A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering: The unsolved challenge,” Bone and Joint Res., vol. 7, no. 3. pp. 232–243, 2018.
F. M. Chen and X. Liu, "Advancing biomaterials of human origin for tissue engineering," Prog. Polym. Sci., vol. 53, pp. 86–168, 2016.
A. S. Motamedi, H. Mirzadeh, F. Hajiesmaeilbaigi, S. Bagheri-Khoulenjani, and M. Shokrgozar, “Effect of electrospinning parameters on morphological properties of PVDF nanofibrous scaffolds,” Prog. Biomater., vol. 6, no. 3, pp. 113–123, 2017.
B. Dhandayuthapani, Y. Yoshida, T. Maekawa, and D. S. Kumar, "Polymeric scaffolds in tissue engineering application: A review," Int. J. Polym. Sci., vol. 2011, pp. 1–19, 2011.
M. Sadat-Shojai, M. T. Khorasani, E. Dinpanah-Khoshdargi, and A. Jamshidi, "Synthesis methods for nanosized hydroxyapatite with diverse structures," Acta Biomater., vol. 9, no. 8, pp. 7591–7621, 2013.
U. Vijayalakshmi, “Preparation of Ag doped hydroxyapatite-Fe3O4-chitosan composites: In vitro biocompatibility study on MG-63 cells for orthopedic applications,” Adv. Sci. Lett., vol. 24, no. 8, pp. 5901–5906, 2017.
W. Damayanti, E. Rochima, and Z. Hasan, “Application of chitosan as antibacterial for pangasius fillet at low temperature storage,” J. Pengolah. Has. Perikan. Indones., vol. 19, no. 3, pp. 321–328, 2017.
U. Anjaneyulu, B. Priyadarshini, A. N. Grace, and U. Vijayalakshmi, “Fabrication and characterization of Ag doped hydroxyapatite-polyvinyl alcohol composite nano fibers and its in vitro biological evaluations for bone tissue engineering applications,” J. Sol-Gel Sci. Technol., vol. 81, no. 3, pp. 750–761, 2016.
F. Sun, H. Zhou, and J. Lee, “Various preparation methods of highly porous hydroxyapatite/polymer nanoscale biocomposites for bone regeneration,” Acta Biomaterialia, vol. 7, no. 11. pp. 3813–3828, 2011.
M. A. H. Margareta, A. Fuad, S. A. Ilmiawati, and S. Wonorahardjo, "Sintesa hydroxyapatite (Ca10(PO4)6(OH)2) berbasis batu kapur," J. Peneliti. Fis. Aplikasinya (JPFA), vol. 5, no. 1, pp. 15–20, 2015.
M. Lubis, M. H. S. Ginting, N. F. Dalimunthe, D. M. T. Hasibuan, and S. Sastrodihardjo, "The influence of chicken egg shell as fillers on biocomposite acrylic resin for denture based," in IOP Conf. Ser.: Mater. Sci. Eng., vol. 180, no. 1, 2017, p. 012008.
B. Riyanto and A. Maddu, “Material of hydroxyapatite-based bioceramics from tuna fishbone,” J. Pengolah. Has. Perikan. Indones., vol. 16, no. 2, pp. 119–132, 2014.
D. Mehta, P. Mondal, V. K. Saharan, and S. George, “Synthesis of hydroxyapatite nanorods for application in water defluoridation and optimization of process variables: Advantage of ultrasonication with precipitation method over conventional method,” Ultrason. Sonochem., vol. 37, pp. 56–70, 2017.
M. Rana, N. Akhtar, S. Rahman, H. M. Jamil, and S. M. Asaduzzaman, "Extraction of hydroxyapatite from bovine and human cortical bone by thermal decomposition and effect of gamma radiation: A comparative study," Int. J. Complement. Altern. Med., vol. 8, no. 3, pp. 1–10, 2017.
H. L. Jaber and T. A. Kovács, “Preparation and synthesis of hydroxyapatite bio-ceramic from bovine bone by thermal heat treatment,” Epa. - J. Silic. Based Compos. Mater., vol. 71, no. 3, pp. 98–101, 2019.
W. Habraken, P. Habibovic, M. Epple, and M. Bohner, "Calcium phosphates in biomedical applications: materials for the future?," Mater. Today, vol. 19, no. 2, pp. 69–87, 2016.
M. H. Alves, B. E. Jensen, A. A. Smith, and A. N. Zelikin, "Poly (vinyl alcohol) physical hydrogels: New vista on a long serving biomaterial," Macromolecular Biosci., vol. 11, no. 10, pp. 1293–1313, 2011.
I. K. Januariyasa, I. D. Ana, and Y. Yusuf, “Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering,” Mater. Sci. Eng. C, vol. 107, p. 110347, 2019.
A. Hasan et al., "Electrospun scaffolds for tissue engineering of vascular grafts," Acta Biomater., vol. 10, no. 1, pp. 11–25, 2014.
R. Stepanyan et al., “Nanofiber diameter in electrospinning of polymer solutions: Model and experiment,” Polymer (Guildf)., vol. 97, pp. 428–439, 2016.
X. Li et al., "Nanostructured scaffolds for bone tissue engineering," J. Biomed. Mater. Res. Part A, vol. 101, no. 8, pp. 2424–2435, 2013.
S. Soliman et al., "Controlling the porosity of fibrous scaffolds by modulating the fiber diameter and packing density," J. Biomed. Mater. Res. Part A, vol. 96, no. 3, pp. 566–574, 2011.
Copyright (c) 2021 Hartatiek Hartatiek, Nasikhudin Nasikhudin, Abdul Aziz Dwi Putra, Yudyanto Yudyanto
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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License