Identifikasi Fitosterol dengan Kromatografi Gas –Spektrometer Massa pada Ekstrak Kloroform Biji Buah Atung (Parinarium GlaberimumHassk) Asal Kabupaten Seram Bagian Barat, Maluku

Candra Yulius Tahya; Elen Tiwery; Mega Gracelia Monaten; Tryani K. Junita Lumbantoruan

doi:10.17977/um0260v4i12020p014

Identifikasi Fitosterol dengan Kromatografi Gas –Spektrometer Massa pada Ekstrak Kloroform Biji Buah Atung (Parinarium GlaberimumHassk) Asal Kabupaten Seram Bagian Barat, Maluku

Candra Yulius Tahya, Elen Tiwery, Mega Gracelia Monaten, Tryani K. Junita Lumbantoruan

Abstract

Five main compounds have been identified in the chloroform extract of Atung fruit seeds (Parinarium glaberimum Hassk) which are linoleic acid (8.88%); stearic acid (8.23%); stigmasta-5,22-dien-3-ol,(3β,22E) or stigmasterol (10.37%); stigmast-5-en-3-ol,(3β) or β-sitosterol (19.59%); and stigmast-5-en-3-ol, (3β, 24S) or γ-sitosterol ( 33.74%) through gas chromatography-mass spectroscopy by analyzing the similarity of molecular weight and positive ion mass/charge fragments and based on the comparison of the GC-MS retention time of phytosterols. This study is the first to report the presence of stigmasterol, β-sitosterol and γ-sitosterol in the chloroform extract of Atung fruit seeds (Parinarium glaberimum Hassk). γ -Sitosterol, the epimer of β-sitosterol, has the greatest abundance in the chloroform extract of Atung fruit seeds. Phytosterol compounds namely stigmasterol, β-sitosterol, and γ-sitosterol have been reported to have many benefits such as reducing blood cholesterol levels, antioxidants, anti-diabetic, anti-inflammatory, and anti-cancer.

Keywords

Atung, Chloroform Extract, GC-MS, Phytosterol, γ-Sitosterol

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References

Aboobucker, S. I., & Suza, W. P. (2019, March). Why Do Plants Convert Sitosterol to Stigmasterol? Frontiers in Plant Scinece, 1-8.

Abu-lafi, S., Rayan, M., Masalha, M. A.-F., Al-Jaas, H., Abu-lafi, M., & Rayan, A. (2019). Phytochemical Composition and Biological Activities of Wild Scolymus maculatus L. Medicines, 6(52), 1-11.

Balamurungan, R., Duraipandiyan, V., & Ignacimuthu, S. (2011). Antidiabetic activity of gamma-sitosterol isomated from Lippia nodiflora L. in streotozotocin induced diabetic rats. European Journal of Pharmacology, 667, 410-418.

Bolanos-Carrillo, M. A., Ventura-Gallegos, J. L., Saldivar-Jimenez, A. D., Zentella-Dehesa, A., & Martinez-Vazquez, M. (2015). Effect of Sterol Isolated from Myrtillocactus geometrizans on Growth Inhibition of Colon and Breast Cancer Cells. Evidence-Based Complementary and Alternatiive Medicine, 1-9.

Endrini, S., Rahmat, A., Ismail, P., & Taufiq-Yap, Y. H. (2014). Cytotoxix effect of gamma-sitosterol from ejibeling (strobilanthes crispus) and its mechanisme of action toward c-myc gene expression and apoptotic pathway. Medical Journal of Indonesia, 23(4), 203-208.

Ferdinandus, M. F., Ufie, C., & Liubana, S. (2017). Karakteristik Lahan Konstruksi Tabel Kebutuhan bagi Pengembangan Atung (Parinarium galberimum Hassk) di Negeri Haruku. Jurnal Budidaya Pertanian, 13(1), 42-48.

Fraile, L., Crisci, E., Cordoba, L., Ferrando, M. A., Osada, J., & Montoya, M. (2012). Immunomodulatory properties of Beta-sitosterol in Pig immune Responses. International Immunopharmacology, 13(3), 316-321.

Hernandez-Hernandez, A., Alarcon-Aguilar, F. J., Jimenez-Estrada, M., Hernandez-Portilla, L. B., Flores-Ortiz, C., & Rodriguez-Monroy, M. A. (2016). Biological Properties and Chemical Composition of Jatropha neopauciflora Pax. African Journal of Traditional, Complementary, and Alternative Medicines, 14(1), 32-42.

Hiariey, S. L. (2013). Ekstraksi biji Atung (Parinarium glaberimum Hassk) untuk mendapatkan bahan pengawet alami dan aplikasinya pada pengasapan filet ikan Tongkol (Euthynnus affinis). Intitut Pertanian Bogor.

Hiariey, S., Pujantoro, L., & Sugiyono. (2013, Agustus). Water extraction of Atung (Parinarium galberimum Hassk) to Obtain a Natural Preservative. Jurnal Keteknikan Pertanian, 49-52.

Kalsait, R. P., Khedekar, P. B., Saoji, A. N., & Bhusar, K. P. (2011). Isolation of phytosterols and antihyperlipiddemic activity of Lagenaria siceraria. Pharmacal Research, 1599-1604.

Kaur, N., Chaudhary, J., Jain, A., & Kishore, L. (2011). Stigmasterol: A Comprehensive Review. International Journal of Pharmaceutical Sciences and Research, 2(9), 2259-2265.

Kim, S.-K., & Ta, V. Q. (2011). Chapter 14 - Potential Beneficial Effects of Marine Algal Sterols on Human Health. Advances in Food and Nutrition Research, 64, 191-198.

Kosma, D. K., Molina, I., & Rowland, O. (2015). GC-MS-Based Analysisi of Chloroform Extracted Suberin-Associated Root Waxes from Arabidopsisand Other Plant Species. Bio-protocol, 5(24), e-1679.

Liu, R., Hao, D., Xu, W., Li, J., Li, X., Shen, D., et al. (2019). B-sitosterol modulates macrophages polazation and attenuates rheumatoid inflmation in mice. Pharamaceutical Biology, 161-168.

Matinahoru, J. M. (2010). Beberapa Aspek Botani, Ekologi dan Silvikurltur Tumbuha Atong (Parinarium glaberimum, Hassk). Fakultas Pertanian. Universitaas Pattimura.

Mohiharapon, T., & Mohiharapon, A. (2015). Application of Atung (Parinarium glaberimum, HASSK) in Handleing of Fresh Pelagis Fish. International Symposium on Aquatic Product Processing 2013 (hal. 70-76). KnE Life Sciences.

Moniharapon, E., Abdegaleil, S. A., Moniharapon, T., Watanabe, Y., & Hashinaga, F. (2004). Purification and Identification of Antibacterial Compound of Atung (Parinarium Glaberimum Hassk) Seed. Pakistan Journal of Biological Science, 7(10), 1667-1670.

Moniharapon, T., Pattipeilohy, F., Mailoa, M. N., & Souhotta, L. M. (2019). Aplikasi PEngawet Alami Atung (Parinarium glaberimum, Hassk) pada Industri Tuna Loin di Dusun Parigi Desa Wahai. Majalah Biam, 70-76.

Moniharapon, T., Pattipeilohy, F., Sormin, R. B., & Gaspersz, F. F. (2006). Aplikasi Penggunaan Atung (Parinarium glaberimum Hassk) pada Penanganan PAska Tangkap dan Bahan Baku Ikan Olahan di Maluku. Ambon: Universitas Pattimura.

Nariya, P. B., Shukla, V. J., Acharya, R. N., Nariya, M. B., Bhatt, P. V., & Tada, R. (2014). Isolation and Characterization of Phytosterol from Cordia macleodii (HOOK F. and Thomson) Bark ny Chromatographic and Spectroscopic method. Asian Journal of Pharmaceutical and Clinical Research, 7(5).

Novontny, L., Abdel-Hamid, M. E., & Hunakova, L. (2017). Anticancer Potential of B-Sitosterol. Intenational Journal of Clinical Pharmacology & Pharmacotherapy, 2(129), 1-5.

Ongkers, V. J. (2017). Efek antidiare infusa biji atung (Parinarium Glaberimum Hassk) pada mencit berina galur swiis dengan metode transit intestinal. Farmasi. Sanata Dharma University.

Sermakkani, M., & Thangapadian, V. (2012). GC_MS Analysis of Cassia Italica Leaf Methanol Extract. Asian Journal of Pharmaceutical and Clinical Research, 5(2), 90-94.

Sheng, Y., & Chen, X.-B. (2009). Isolation and identification of an isomer of B-sitosterol by HPLC and GC-MS. Health, 1(3), 203-206.

Shi, C., Liu, J., Wu, F. Z., Yew, D. T., & Xu, J. (2011, December). B-sitosterol Inhibits High Cholesterol-Induced Platelet B-amyloid Release. J Bioenerg Biomembr, 43(6), 691-697.

Sirikhansaeng, P., Tanee, T., Sudmoon, R., & Chaveerach, A. (2017). Major Phytochemical as Gamma-Sitosterol Disclosing and Toxicity Testing in Lagerstroemia. Evidance-Based Complementary and Alternative Medicine, 1-10.

Sundarraj, S., Thangam, R., Sreevani, V., Kaveri, K., Gunasekaran, P., Achiraman, S., et al. (2012). Gamma-sitosterol from Acacia Nilotica L. Induces G2/M Cell Cycle Arrest and Apoptosis Through c-Myc Supression in MCF-T and A549 Cells. J Ethnopharmacol, 141(3), 803-809.

Ward, M. G., Li, G., Barbosa-Lorenzi, V. C., & Hao, M. (2017, Agustus). Scientific Reports, 1-13.

Zhen, Z.-H., Quan, Y.-C., Jiang, H.-Y., Wen, Z.-S., Qu, Y.-l., & Guan, L.-P. (2015). Fucosterol, a Sterol Extracted from Sargassum Fusiforme, Shows Antidepressant and Anticovulsant Effect. Eur J Pharmacol, 131-138.

DOI: http://dx.doi.org/10.17977/um0260v4i12020p014